Found this nifty site where A/P Chen Ken-Shiung might have based his practical on.
I've yet to read thru it in detail, but it seems to cover:
- technical aspects of Real Time PCR
- Quantification of mRNA levels using Real Time PCR Standard Curve Mehtod
- Interpretation of the Tm Curves
- Further Deriavations of the Tm Curves (i.e. log scale etc)
Alot of the graphs he showed us during the pract seemed to pulled right out of here...
On my knees
12 years ago
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